'3D imaging after clearing: theory and applications'

Combining whole-organ immunohistochemistry with tissue-clearing and light-sheet laser-scanning ultramicroscopy, we study the development and 3D organization of the hypothalamic-pituitary-gonadal axis in mammals.  In his talk, novel data will be presented to show how this technique can be adapted to human tissues for volume imaging during fetal development as well as in adult post-mortem tissues. These techniques open a novel route for high-resolution imaging studies of brain architecture in mammals in physiological and pathological conditions.

About

Paolo Giacobini is a basic scientist focused on neurodevelopmental events leading to the correct maturation and function of the mammalian reproductive axis. He obtained his PhD in Neuroscience in 2005 at the University of Turin, Italy. This was a graduate partnership program between the University of Turin and the National Institute of Health (NIH), Bethesda (USA), where he spent several years during his PhD and post-doctoral formation. In 2009 he was recruited as a tenured research scientist and group leader at the French National Institute of Health and Medical Research (INSERM) in Lille, France. In 2016 he was awarded an ERC-Consolidator Grant that is focused on the central neuroendocrine abnormalities of infertility and development of new potential therapeutic strategies.