Droplet Digital PCR: ddPCR por multiplex razones

More than 11,900 scientific publications and 109 clinical studies have validated Bio-Rad Laboratories’ Droplet Digital PCR (ddPCR) technology in research, clinical diagnostics, animal health, and food and biopharmaceutical quality control, demonstrating its utility since its launch over a decade ago.
As a third-generation PCR technology, ddPCR offers absolute quantification with exceptional sensitivity and precision of DNA, RNA, or live cells in low-abundance or highly complex samples, without the need for a standard curve.

In this in-person seminar, discover:

• The power of nanodroplet partitioning and the advantages of the technology compared to qPCR or NGS.
• The most important applications of Droplet Digital PCR technology:
  • Detection and quantification of rare events/mutations.
  • Copy Number Variation (CNV) and linkage analysis.
  • Detection and quantification of gene editing.
  • Gene expression analysis in mRNA, miRNA, lncRNA, and single-cell formats.
  • Evaluation of methylation patterns.
  • Integration with the NGS workflow.
• Experimental optimisation and Droplet Digital PCR assay design.
• Workflow: QX systems, consumables, assays, and kits.

Certificates of attendance will be provided upon request at cimus.xestion [at] usc.es (cimus[dot]xestion[at]usc[dot]es). Please do not forget to enter your name and surname in the printout given during the lecture.