Modelling thyroid hormone action in human cerebral organoids using single cell omics
Robert Opitz
Principal Investigator, Endocrine regulation of human brain development Group. Department of Pediatric Endocrinology & Diabetology, Charité-Universitätsmedizin Berlin (Germany)
Theatre room, CiMUS
About
hiPSC-derived cerebral organoids recapitulate key aspects of first-trimester human cortical neurogenesis. Using this technology, we modelled the regulatory role of thyroid hormones (TH) during human corticogenesis by immunofluorescence, RNAscope and single cell multiomics (RNA-seq, ATAC-seq, CUT&Tag). The talk will discuss a developmental timer function of TH, promotional effects on upper-layer neuron generation and TH effects on the pace of neuronal differentiation and maturation.
Bio
For over 25 years, Dr Opitz's research interests centered around developmental aspects of the thyroid hormone system including experimental work in Xenopus tadpoles (PhD thesis), zebrafish embryos and stem cell-derived organoids (post-doc). At Charité Berlin, his group now uses single cell technologies and cerebral organoid models to investigate the role of thyroid hormones during human brain development.
Hosted by PI Clara V. Álvarez. Neoplasia & Endocrine Differentiation group, CiMUS
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