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Molecular Medicine Programme | Cancer

Stem Cells and Human Diseases (SC&HD)

Miguel Fidalgo
Group Leader | Associate Professor
Field of knowledge

Pluripotent stem cells (PSCs), including embryo-derived ESCs and somatic cell-derived iPSCs, possess unlimited self-renewal potential in culture and the ability to differentiate into all cell types of the adult organism. Given these unique cellular properties, PSCs are not only considered a major promise for regenerative medicine but also a valuable tool to interrogate molecular underpinnings controlling cell fate determination, and a powerful platform to uncover altered molecular mechanisms linked to human disease.

Research Lines

Our global aim is focused on understanding how regulatory information encoded by the genome is integrated with the epigenetic and epitranscriptomic machineries to control cellular plasticity in the context of cellular reprogramming and pluripotency, and how perturbations of these mechanisms could be associated with defects in development and disease. 

Some of the ongoing questions we are interested in studying are: 

  • Which molecular programs are in place to unleash the full potential of pluripotent cells for therapeutic purposes? 
  • How do dietary perturbations lead to aberrant epigenetic and metabolic regulation which compromise the generation of safe iPSCs from elderly donors? 
  • Can we modulate the impact of obesity and aging in induced reprogramming technology to achieve cellular rejuvenation without affecting cell identity? 

    For this purpose, we are employing a broad combination of methods of cell and molecular biology, genome interventions, high-throughput genomic and proteomic approaches, and bioinformatics. 

    For additional information on our group, please visit the website:


Helena Covelo Molares
Margarita Salas Postdoctoral Fellowship


Cristina Ameneiro Quiñoy
Research collaborator
Adriana Escudero Pérez
FPU fellowship
Vera García Outeiral
FPU fellowship
Tiago Manuel Martins Moreira
PhD student
David Martínez Delgado
PhD student
Celia Fernández Rial
PhD student

Selected publications

Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells.

Fuentes-Iglesias A., Ameneiro C., Guallar D., Fidalgo M.

An Optimized Immunoprecipitation Protocol for Assessing Protein-RNA Interactions In Vitro.

Fuentes-Iglesias A, Garcia-Outeiral V, Pardavila JA, Wang J, Fidalgo M, Guallar D.

ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress

Diana Guallar, Alejandro Fuentes-Iglesias, Yara Souto, Cristina Ameneiro, Oscar Freire-Agulleiro, Jose Angel Pardavila, Adriana Escudero, Vera Garcia-Outeiral, Tiago Moreira, Carmen Saenz, Heng Xiong, Dongbing Liu, Shidi Xiao, Yong Hou, Kui Wu, Daniel Torrecilla, Jochen C. Hartner, Miguel G. Blanco, Leo J. Lee, Miguel López, Carl R. Walkley, Jianlong Wang, Miguel Fidalgo.

BMAL1 coordinates energy metabolism and differentiation of pluripotent stem cells

Ameneiro, Cristina; Moreira, Tiago; Fuentes-Iglesias, Alejandro; Coego, Alba; Garcia-Outeiral, Vera; Escudero, Adriana; Torrecilla, Daniel; Mulero-Navarro, Sonia; Carvajal-Gonzalez, Jose Maria; Guallar, Diana; Fidalgo, Miguel
RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells
Guallar, Diana; Bi, Xianju; Pardavila, Jose Angel; Huang, Xin; Saenz, Carmen; Shi, Xianle; Zhou, Hongwei; Faiola, Francesco; Ding, Junjun; Haruehanroengra, Phensinee; Yang, Fan; Li, Dan; Sanchez-Priego, Carlos; Saunders, Arven; Pan, Feng; Valdes, Victor Julian; Kelley, Kevin; Blanco, Miguel G.; Chen, Lingyi; Wang, Huayan; Sheng, Jia; Xu, Mingjiang; Fidalgo, Miguel; Shen, Xiaohua; Wang, Jianlong

Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States.

Fidalgo M, Huang X, Guallar D, Sanchez-Priego C, Valdes VJ, Saunders A, Ding J, Wu WS, Clavel C, Wang J.

Selected Results